Fumigation with ?14 g m?3 ozone for 1 h at 3 ?C controls Latrodectus hesperus

Ozone fumigations were evaluated for postharvest control of Western black widow spider (BWS), Latrodectus hesperus (Chamberlin and Ivie), in fresh table grapes destined for export from California USA. Mature adult female black widow spiders were contained in separate gas-permeable cages within a flow-through vacuum chamber and exposed for 1 h at 3 ? 1 ?C (View the MathML source ? s) and 67.5 kPa to gaseous ozone at eight steady-state concentrations (i.e, [O3]ss) over the range 0.71 ? 0.04?16.91 ? 0.28 g m?3 (View the MathML source ? s) (i.e., 500 ? 25?12,000 ? 200 ppmv (?L L?1)) with or without supplementation of atmospheric carbon dioxide levels, respectively 129.3 or ca. 0.8 g m?3 [CO2]ss (i.e., 100,000 or ca. 600 ppmv). Regression models of the concentration-mortality response predict 98% mortality of adult female BWS populations following ozone fumigation for 1 h at 3 ? 1 ?C when headspace [O3]ss is maintained at ?14 g m?3 (i.e., 10,000 ppmv) with ambient atmospheric CO2 levels ([CO2]ss = 0.8 g m?3). Providing evidence to support the use of these treatment parameters for control of BWS in packed table grapes, 0 survivors were observed from 268 total specimens treated in a series of confirmatory fumigations conducted for 1 h at 3 ? 1 ?C and 67.5 kPa with headspace [O3]ss and [CO2]ss maintained ?14 and ca. 0.8 g m?3, respectively. SourcesPostharvest fumigation of California table grapes with ozone to control Western black widow spider (Araneae: Theridiidae)Spencer S. Walse, J. Steven Tebbets, James G. LeeschUSDA, Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Avenue, Parlier, CA 93648-9757, United StatesPostharvest Biology and Technology,?Volume 134, December 2017, Pages 1?4 Picture by vivirmejor.com ?