Melatonin significantly increased glucoraphanin content and myrosinase activity in fresh-cut broccoli?

The effect of postharvest melatonin treatment on sulforaphane production of fresh-cut broccoli at 4? during storage was investigated in this study. Florets treated with 100 ?M melatonin exhibited higher contents of total glucosinolates and sulforaphane. Glucoraphanin content was significantly increased after melatonin treatment, and which was explained by gene analysis. Expressions of glucoraphanin biosynthesis genes including Elong, CYP83A1, MYB28, UGT74B1 and FMOGS-OX1 were up-regulated while AOP2 was obviously decreased by melatonin treatment, leading to a higher glucoraphanin accumulation. In addition, application of melatonin enhanced the myrosinase activity and the expression level of MYO, benefiting the formation of sulforaphane. This study demonstrates that melatonin treatment positively affected the glucoraphanin - sulforaphane system in postharvest fresh-cut broccoli. SourcesMelatonin treatment affects the glucoraphanin-sulforaphane system in postharvest fresh-cut broccoli (Brassica oleracea L.)Liyang Wei, Changhong Liu, Huanhuan Zheng & Lei Zhenghttps://doi.org/10.1016/j.foodchem.2019.125562Food Chemistry,?Available online 30 September 2019, 125562In Press, Journal Pre-proofhttps://www.sciencedirect.com/science/article/pii/S0308814619316863Picture by?Fructiban