The effect of 1-methylcyclopropene (1-MCP) treatment on superficial color, sugar content and the activities of sugar metabolism enzymes, the expression of sucrose transporters and carbohydrate metabolizing enzymes in broccoli florets were investigated. Broccoli florets treated with 2.5 ?L L?1 of 1-methylcyclopropene (1-MCP) inhibited the increase of lightness (L*)
The effect of 1-methylcyclopropene (1-MCP) treatment on superficial color, sugar content and the activities of sugar metabolism enzymes, the expression of sucrose transporters and carbohydrate metabolizing enzymes in broccoli florets were investigated. Broccoli florets treated with 2.5 ?L L?1 of 1-methylcyclopropene (1-MCP) inhibited the increase of lightness (L*) value and retarded the decrease in hue angle (H) value and chlorophyll content. This treatment maintained higher levels of sugars comparing with control florets. In general, the activities of sucrose synthase-synthesis direction (SS-S) were enhanced in florets treated with 1-MCP, whereas the activities of sucrose synthase-cleavage direction (SS-C), glucokinase (GK) and UDP-glucose pyrophosphorylase (UGPase) were reduced. In addition, the expression of genes encoding sucrose transporters (BoSUC1 and BoSUC2) and carbohydrate metabolizing enzymes (BoINV1, BoHK1 and BoHK2) was induced upon 1-MCP treatment. These results indicated that 1-MCP can delay senescence of broccoli florets in the present study may be attributed to maintaining higher sugar content through regulation of sugar metabolism.SourcesEffect of 1-methylcyclopropene on senescence and sugar metabolism in harvested broccoli florets??? Feng Xua (b), Hongfei Wang (b), Yuechang Tang (b), Shuanquan Dong (b), Xing Qiao (b), Xuehong Chen (c), Yonghua Zheng (a) a College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR Chinab Department of Food Science and Engineering, Ningbo University, Ningbo 315211, Zhejiang, PR Chinac College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, Jiangsu, PR ChinaPostharvest Biology and TechnologyPostharvest Biology and Technology, Volume 116, June 2016, Pages 45?49 The picture is by comida.dibujos.net