EIS enables to quantify fermentable sugars from pineapple waste in an easy way

Electrochemical Impedance Spectroscopy (EIS) has been used to develop a methodology able to identify and quantify fermentable sugars present in the enzymatic hydrolysis phase of second-generation bioethanol production from pineapple waste. Thus, a low-cost non-destructive system consisting of a stainless double needle electrode associated to an electronic equipment that allows the implementation of EIS was developed. In order to validate the system, different concentrations of glucose, fructose and sucrose were added to the pineapple waste and analyzed both individually and in combination. Next, statistical data treatment enabled the design of specific Artificial Neural Networks-based mathematical models for each one of the studied sugars and their respective combinations. The obtained prediction models are robust and reliable and they are considered statistically valid (CCR% >93.443%). These results allow us to introduce this EIS-based technique as an easy, fast, non-destructive, and in-situ alternative to the traditional laboratory methods for enzymatic hydrolysis monitoring.More informationSourcesAn Electrochemical Impedance Spectroscopy-Based Technique to Identify and Quantify Fermentable Sugars in Pineapple Waste Valorization for Bioethanol ProductionClaudia Conesa 1,*, Eduardo Garc?a-Breijo 2, Edwin Loeff 2, Luc?a Segu? 1, Pedro Fito 1 andNicol?s Laguarda-Mir? 2,*1 Instituto de Ingenier?a de Alimentos para el Desarrollo (IIAD), Universitat Polit?cnica de Val?ncia,Cam? de Vera s/n, 46022 Valencia, Spain; E-Mails: lusegil@upvnet.upv.es (L.S.);pfito@tal.upv.es (P.F.)2 Centro de Reconocimiento Molecular y Desarrollo Tecnol?gico (IDM), Unidad Mixta UniversitatPolit?cnica de Val?ncia, Universitat de Val?ncia, Cam? de Vera s/n, 46022 Valencia, Spain;E-Mails: egarciab@eln.upv.es (E.G.-B.); edloe@upvnet.upv.es (E.L.)* Authors to whom correspondence should be addressed; E-Mails: clcodo@upvnet.upv.es (C.C.);nilami@iqn.upv.es (N.L.-M.); Tel.: +34-963877007 (ext. 83626) (C.C.);+34-963877007 (ext. 71848) (N.L.-M.); Fax: +34-963877189 (N.L.-M.).Academic Editor: Alexander StarSensors 2015, 15, 22941-22955; doi:10.3390/s150922941Picture by bits.technohyst.com